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100x minimum essential medium-non-essential amino acid (mem-neaa) solution  (Thermo Fisher)


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    Thermo Fisher 100x minimum essential medium-non-essential amino acid (mem-neaa) solution
    100x Minimum Essential Medium Non Essential Amino Acid (Mem Neaa) Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimum+essential+medium+non-essential+amino+acids+solution+(mem-neaa/pmc11211886-148-40-51
    Average 90 stars, based on 1 article reviews
    100x minimum essential medium-non-essential amino acid (mem-neaa) solution - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson’s disease in human dopaminergic neurons and a mouse model
    Article Snippet: Minimum Essential Medium (MEM) Non-Essential Amino Acids Solution (NEAA) , Gibco , Cat#11140050.

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: Retinoic acid signalling inhibits myogenesis by blocking MYOD translation in pig skeletal muscle cells.
    Article Snippet: Re-suspended cell pellets with skeletal muscle stem cell proliferation medium containing 20% foetal bovine serum (FBS, WenRen, Shanghai, China), 0.5% chicken embryo extract (CEE, Genimi, USA), 1% GlutaMax (Gibco, Grand Island, NY, USA), 1% Minimum Essential Medium Non-Essential Amino Acids Solution (MEM-NEAA, Gibco, Grand Island, NY, USA), 1% penicillin–streptomycin (Gibco, Grand Island, NY, USA), 2.5 ng/ml basic fibroblast growth factor (bFGF, Gibco, Grand Island, NY, USA), Roswell Park Memorial Institute 1640 medium (RPMI 1640, Gibco, Grand Island, NY, USA).

    Article Title: SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson's disease in human dopaminergic neurons and a mouse model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human/mouse fibroblast growth factor (FGF)-8b R&D system Cat#423-F8-025/CF CHIR99021 STEMGENT Cat#04-0004-02 DMEM Gibco Cat#11965092 Fetal bovine serum Gibco Cat#26140079 Penicillin‒streptomycin Gibco Cat#15140122 Human a-Syn monomers Proteos Cat#RP-003 NovexTM WedgeWellTM Invitrogen Cat#XP08165BOX Coomassie R-250 Thermo Fisher Scientific Cat#20278 TRIzol Invitrogen Cat#15596018 HiSecriptTM RH( ) RT Premix Kit Intron Biotechnology Cat#25087 iQ SYBR Green Supermix Bio-Rad Cat#1708882 TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R Illumina Cat#20040525 Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) Roche Cat#11684795910 Tween 20 Sigma‒Aldrich Cat#TR1027-500-00 40,6-diamidino-2-phenylindole (DAPI) Cat#D9542-1MG Alamar blue Invitrogen Cat#DAL1025 Zoletil Virbac Cat#212604 Rompun Bayer Cat#BAY1470 Optimal cutting temperature (O.C. compound) Sakura Finetek Inc Cat#4583 Triton Biosesang Cat#TR1027-500-00 Sodium azide Sigma‒Aldrich Cat#447810250 Normal Goat serum Jackson ImmunoResearch Laboratories Cat#005-000-121 ABC reagents Vector Laboratories, Inc. Cat#PK-6101, Cat#PK-6102 SIGMAFAST 3,3-Diaminobenzidine tablets Cat#D4293 Vector SG Substrate Kit, Peroxidase (HRP) Vector Laboratories Cat#SK-4700 HaltTM Protease and Phosphatase Inhibitor Single-Use Cocktail Thermo Scientific Cat#1861281 Bicinchoninic Acid (BCA) Protein Assay Kit Thermo Scientific Cat#23225 2X Laemmli buffer Bio-Rad Cat#1610737 b-mercaptoethanol Biosesang Cat#MR1015-100-00 Nitrocellulose (NC) membranes Bio-Rad Cat#162-0115 Chemiluminescence (ECL) Pierce Cat#34580 Critical commercial assays Lysosomal Intracellular Activity Assay Kit abcam Cat#ab234622 Proteasome assay kit Cayman chemical Cat#10008041 AlamarBlueTM Cell Viability Reagent Invitrogen Cat#DAL1100 In Situ Cell Death Detection Kit, Fluorescein Roche Cat#11684795910 Deposited data Bulk RNA sequencing data https://www.ncbi.nlm.nih.gov/geo/ Accession no. GSE211934 Experimental models: Cell lines H9 WiCell Cat#WA09 (lot:WB0143) Vero-E6 cells ATCC Cat#CRL-1586 (Continued on next page) Cell Reports Medicine 5, 101570, May 21, 2024 e3 OPEN ACCESS

    Article Title: Preclinical pharmacokinetic characterization of amdizalisib, a novel PI3Kδ inhibitor for the treatment of hematological malignancies
    Article Snippet: Fetal bovine serum (FBS), 1X high-glucose Dulbecco’s Modified Eagle Medium (DMEM), 0.05% (1X) trypsin–EDTA solution, 1X Hank’s balanced salt solution (HBSS), 1 M (100X) N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), 100X penicillin–streptomycin solution, 100 mM (100X) sodium pyruvate, 200 mM (100X) L-glutamine, and 100X minimum essential medium-non-essential amino acid (MEM-NEAA) solution were obtained from Gibco (Invitrogen, Carlsbad, CA, United States).

    Knock-Out:

    Article Title: Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration
    Article Snippet: On day 0, iPSC colonies were separated into single cells by enzymatic dissociation with 0.25% trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) after treatment with 10 μM Y27632 (Nacalai Tesque, Kyoto, Japan) for 30 minutes. .. To form the aggregation of iPSCs, 12 000 cells were plated in each well of an ultra-low attachment 96-well plate with V-bottomed conical wells (Sumitomo Bakelite, Tokyo, Japan) in 100 μL aggregation medium containing Glasgow’s Minimum Essential Medium (Sigma-Aldrich, St.Louis, MO, USA), 20% [v/v] KnockOut serum replacement (Thermo Fisher Scientific), 1% [v/v] Minimum Essential Medium Non-essential Amino Acid Solution (MEM-NEAA) (Thermo Fisher Scientific), 1 mM sodium pyruvate (Thermo Fisher Scientific), 100 U/mL penicillin, 100 μg/mL streptomycin, 250 ng/mL amphotericin B (Thermo Fisher Scientific), 1 μM 2-mercaptoethanol (Wako, Osaka, Japan), 3 μM IWR1-endo (Wako), 100 nM LDN-193189 (Stemgent, MA, USA), 5 μM SB431542 (Stemgent), and 30 μM Y27632 (Nacalai Tesque). .. On day 2, 16 aggregates were transferred along with the medium using a 1000μL micropipette onto 2 types of SiF sheets in an ultra-low attachment 96-well plate (Corning, NY, USA), with the aggregation medium changed twice a day.

    Article Title: SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson’s disease in human dopaminergic neurons and a mouse model
    Article Snippet: The cells were seeded at 1.5 × 10 6 cells per well on Geltrex (#A1569601, Gibco, MA, USA)-coated 6-well plates. .. Cells were grown for 11 days on Geltrex in Dulbecco’s modified Eagle medium (DMEM)/F12 (#11320033, Gibco, MA, USA) with 10% KnockOut Serum Replacement (#10828010, Gibco, MA, USA), Minimum Essential Medium (MEM) Non-Essential Amino Acids Solution (NEAA) (#11140050, Gibco, MA, USA), 2 mM L-glutamine and 10 mM β-mercaptoethanol containing supplements detailed in . .. KSR medium was gradually shifted to N2 (#17502048, Gibco, MA, USA)-containing medium starting on Day 5 of differentiation., , The medium was changed to Neurobasal/B27/L-glut containing medium (NB/B27, #A3653401, Gibco, MA, USA) supplemented detailed in .

    Modification:

    Article Title: SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson’s disease in human dopaminergic neurons and a mouse model
    Article Snippet: The cells were seeded at 1.5 × 10 6 cells per well on Geltrex (#A1569601, Gibco, MA, USA)-coated 6-well plates. .. Cells were grown for 11 days on Geltrex in Dulbecco’s modified Eagle medium (DMEM)/F12 (#11320033, Gibco, MA, USA) with 10% KnockOut Serum Replacement (#10828010, Gibco, MA, USA), Minimum Essential Medium (MEM) Non-Essential Amino Acids Solution (NEAA) (#11140050, Gibco, MA, USA), 2 mM L-glutamine and 10 mM β-mercaptoethanol containing supplements detailed in . .. KSR medium was gradually shifted to N2 (#17502048, Gibco, MA, USA)-containing medium starting on Day 5 of differentiation., , The medium was changed to Neurobasal/B27/L-glut containing medium (NB/B27, #A3653401, Gibco, MA, USA) supplemented detailed in .



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    Journal: Cell Reports Medicine

    Article Title: SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson’s disease in human dopaminergic neurons and a mouse model

    doi: 10.1016/j.xcrm.2024.101570

    Figure Lengend Snippet:

    Article Snippet: Cells were grown for 11 days on Geltrex in Dulbecco’s modified Eagle medium (DMEM)/F12 (#11320033, Gibco, MA, USA) with 10% KnockOut Serum Replacement (#10828010, Gibco, MA, USA), Minimum Essential Medium (MEM) Non-Essential Amino Acids Solution (NEAA) (#11140050, Gibco, MA, USA), 2 mM L-glutamine and 10 mM β-mercaptoethanol containing supplements detailed in .

    Techniques: Recombinant, Binding Assay, Affinity Purification, Virus, Variant Assay, SYBR Green Assay, Sample Prep, TUNEL Assay, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Activity Assay, In Situ, RNA Sequencing Assay, Transgenic Assay, Software